Robust Physiological Metrics From Sparsely Sampled Networks.
Alan A Cohen, Sebastien Leblanc, Xavier Roucou
Front Physiol. 2021 Feb 10;12:624097. doi: 10.3389/fphys.2021.624097.
PMID: 33643068
Potentiation of B2 receptor signaling by AltB2R, a newly identified alternative protein encoded in the human bradykinin B2 receptor gene
Maxime Gagnon, Martin Savard, Jean-François Jacques, Ghassan Bkaily, Sameh Geha, Xavier Roucou, Fernand Gobeil
Jan-Jun 2021;296:100329. doi: 10.1016/j.jbc.2021.100329. Epub 2021 Jan 23.
PMID: 33497625
The FUS gene is dual-coding with both proteins contributing to FUS-mediated toxicity.
Brunet MA, Jacques JF, Nassari S, Tyzack GE, McGoldrick P, Zinman L, Jean S, Robertson J, Patani R, Roucou X.
EMBO Rep. 2020 Nov 23:e50640. doi: 10.15252/embr.202050640. Online ahead of print.
PMID: 33226175
OpenProt 2021: deeper functional annotation of the coding potential of eukaryotic genomes.
Brunet MA, Lucier JF, Levesque M, Leblanc S, Jacques JF, Al-Saedi HRH, Guilloy N, Grenier F, Avino M, Fournier I, Salzet M, Ouangraoua A, Scott MS, Boisvert FM, Roucou X.
Nucleic Acids Res. 2020 Nov 12:gkaa1036. doi: 10.1093/nar/gkaa1036. Online ahead of print.
PMID: 33179748
How to Illuminate the Dark Proteome Using the Multi-omic OpenProt Resource.
Brunet MA, Lekehal AM, Roucou X.
Curr Protoc Bioinformatics. 2020 Sep;71(1):e103. doi: 10.1002/cpbi.103.
PMID: 32780568
Reconsidering proteomic diversity with functional investigation of small ORFs and alternative ORFs.
Brunet MA, Leblanc S, Roucou X.
Exp Cell Res. 2020 Aug 1;393(1):112057. doi: 10.1016/j.yexcr.2020.112057. Epub 2020 May 6.
PMID: 32387289
UBB pseudogene 4 encodes functional ubiquitin variants.
Dubois ML, Meller A, Samandi S, Brunelle M, Frion J, Brunet MA, Toupin A, Beaudoin MC, Jacques JF, Lévesque D, Scott MS, Lavigne P, Roucou X, Boisvert FM.
Nat Commun. 2020 Mar 11;11(1):1306. doi: 10.1038/s41467-020-15090-6.
PMID: 32161257
Optimized Sample Preparation Workflow for Improved Identification of Ghost Proteins.
Cardon T, Hervé F, Delcourt V, Roucou X, Salzet M, Franck J, Fournier I.
Anal Chem. 2020 Jan 7;92(1):1122-1129. doi: 10.1021/acs.analchem.9b04188. Epub 2019 Dec 24.
PMID: 31829555
Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames.
Brunet MA, Roucou X.
J Vis Exp. 2019 Apr 11;(146). doi: 10.3791/59589.
PMID: 31033953
OpenProt: a more comprehensive guide to explore eukaryotic coding potential and proteomes.
Brunet MA, Brunelle M, Lucier JF, Delcourt V, Levesque M, Grenier F, Samandi S, Leblanc S, Aguilar JD, Dufour P, Jacques JF, Fournier I, Ouangraoua A, Scott MS, Boisvert FM, Roucou X.
Nucleic Acids Res. 2018 Oct 9. doi: 10.1093/nar/gky936.
PMID: 30299502
OpenProt: a more comprehensive guide to explore eukaryotic coding potential and proteomes.
Brunet MA, Brunelle M, Lucier JF, Delcourt V, Levesque M, Grenier F, Samandi S, Leblanc S, Aguilar JD, Dufour P, Jacques JF, Fournier I, Ouangraoua A, Scott MS, Boisvert FM, Roucou X.
Nucleic Acids Res. 2018 Oct 9. doi: 10.1093/nar/gky936.
PMID: 30299502
The protein coded by a short open reading frame, not by the annotated coding sequence is the main gene product of the dual-coding gene MIEF1.
Delcourt V, Brunelle M, Roy AV, Jacques JF, Salzet M, Fournier I, Roucou X.
Mol Cell Proteomics. 2018 Sep 4. pii: mcp.RA118.000593. doi: 10.1074/mcp.RA118.000593.
PMID: 30181344
Recognition of the polycistronic nature of human genes is critical to understanding the genotype-phenotype relationship.
Brunet MA, Levesque SA, Hunting DJ, Cohen AA, Roucou X.
Genome Res. 2018 May;28(5):609-624. doi: 10.1101/gr.230938.117.
PMID: 29626081
Deep transcriptome annotation enables the discovery and functional characterization of cryptic small proteins.
Samandi S, Roy AV, Delcourt V, Lucier JF, Gagnon J, Beaudoin MC, Vanderperre B, Breton MA, Motard J, Jacques JF, Brunelle M, Gagnon-Arsenault I, Fournier I, Ouangraoua A, Hunting DJ, Cohen AA, Landry CR, Scott MS, Roucou X.
Elife. 2017 Oct 30;6. pii: e27860. doi: 10.7554/eLife.27860.
PMID: 29083303
Small proteins encoded by unannotated ORFs are rising stars of the proteome, confirming shortcomings in genome annotations and current vision of an mRNA.
Delcourt V, Staskevicius A, Salzet M, Fournier I, Roucou X.
Proteomics. 2017 Jun 19. doi: 10.1002/pmic.201700058.
PMID: 28627015
Death of a dogma: eukaryotic mRNAs can code for more than one protein.
Mouilleron H, Delcourt V, Roucou X.
Nucleic Acids Res. 2016 Jan 8;44(1):14-23.
PMID: 26578573
Found in translation: functions and evolution of a recently discovered alternative proteome.
Landry CR, Zhong X, Nielly-Thibault L, Roucou X.
Curr Opin Struct Biol. 2015 Jun;32:74-80. doi: 10.1016/j.sbi.2015.02.017. Epub 2015 Mar 18.
PMID: 25795211
Regulation of PrP(C) signaling and processing by dimerization.
Roucou X
Front Cell Dev Biol. 2:57. doi: 10.3389/fcell.2014.00057. eCollection 2014. Review.
PMID: 25364762
Development of kinomic analyses to identify dysregulated signaling pathways in cells expressing cytoplasmic PrP
Shott RH, Appanah C, Grenier C, Tremblay G, Roucou X, Schang LM
Virol J. 11:175. doi: 10.1186/1743-422X-11-175
PMID: 25280966
Aß induces its own prion protein N-terminal fragment (PrPN1)-mediated neutralization in amorphous aggregates
Béland M, Bédard M, Tremblay G, Lavigne P, Roucou
Neurobiol Aging. 35(7):1537-48. doi: 10.1016/j.neurobiolaging.2014.02.001
PMID: 24602510
Taking advantage of physiological proteolytic processing of the prion protein for a therapeutic perspective in prion and Alzheimer diseases
Béland M, Roucou X
Prion. 8(1):106-110
PMID: 24335160
Direct detection of alternative open reading frames translation products in human significantly expands the proteome
Vanderperre B, Lucier JF, Bissonnette C, Motard J, Tremblay G, Vanderperre S, Wistorski M, Salzet M, Boisvert FM, Roucou X
PLoS One. 8(8):e70698. doi: 10.1371/journal.pone.0070698
PMID: 23950983
p53 Aggregates penetrate cells and induce the co-aggregation of intracellular p53
Forget KJ, Tremblay G, Roucou X
PLoS One. 8(7):e69242. doi: 10.1371/journal.pone.0069242.
PMID: 23844254
An out-of-frame overlapping reading frame in the ataxin-1 coding sequence encodes a novel ataxin-1 interacting protein
Bergeron D, Lapointe C, Bissonnette C, Tremblay G, Motard J, Roucou X
J Biol Chem. 288(30):21824-21835. doi: 10.1074/jbc.M113.472654
PMID: 23760502
Homodimerization as a molecular switch between low and high efficiency PrP(C) cell surface delivery and neuroprotective activity
Béland M, Roucou X
Prion. 7(2):170-174. doi: 10.4161/pri.23583
PMID: 23357826
Aggregation and neurotoxicity of recombinant a-synuclein aggregates initiated by dimerization
Roostaee A, Beaudoin S, Staskevicius A, Roucou X
Mol Neurodegener. 8:5. doi: 10.1186/1750-1326-8-5
PMID: 23339399
PrP(C) homodimerization stimulates the production of PrPC cleaved fragments PrPN1 and PrPC1
Béland M, Motard J, Barbarin A, Roucou X
J Neurosci. 32(38):13255-13263
PMID: 22993441
HAltORF: a database of predicted out-of-frame alternative open reading frames in human
Vanderperre B, Lucier JF, Roucou X
Database (Oxford). 2012:bas025. doi: 10.1093/database/bas025
PMID: 22613085
The prion protein unstructured N-terminal region is a broad-spectrum molecular sensor with diverse and contrasting potential functions
Béland M, Roucou X
J Neurochem. 120(6):853-868. doi: 10.1111/j.1471-4159.2011.07613.x. Review.
PMID: 22145935
An overlapping reading frame in the PRNP gene encodes a novel polypeptide distinct from the prion protein
Vanderperre B, Staskevicius AB, Tremblay G, McCoy M, O'Neill MA, Cashman NR, Roucou X
FASEB J. 25(7):2373-2386. doi: 10.1096/fj.10-173815
PMID: 21478263
Aggregation and amyloid fibril formation induced by chemical dimerization of recombinant prion protein in physiological-like conditions
Roostaee A, Côté S, Roucou X
J Biol Chem. 284(45):30907-30916. doi: 10.1074/jbc.M109.057950
PMID: 19710507
Prion protein and RNA: a view from the cytoplasm
Roucou X
Front Biosci (Landmark Ed). 14:5157-5164. Review
PMID: 19482610
Reconstitution of chromatoid body-like particles in cultured cells: a novel approach to elucidate the mechanism of assembly and function of the chromatoid body
Roucou X
RNA Biol. 6(2):165-168. Review
PMID: 19229140
A large ribonucleoprotein particle induced by cytoplasmic PrP shares striking similarities with the chromatoid body, an RNA granule predicted to function in posttranscriptional gene regulation
Beaudoin S, Vanderperre B, Grenier C, Tremblay I, Leduc F, Roucou X
Biochim Biophys Acta. 1793(2):335-345. doi: 10.1016/j.bbamcr.2008.10.009
PMID: 19014979
Aggresomes do not represent a general cellular response to protein misfolding in mammalian cells
Beaudoin S, Goggin K, Bissonnette C, Grenier C, Roucou X
BMC Cell Biol. 9:59. doi: 10.1186/1471-2121-9-59
PMID: 18937858
Prion protein aggresomes are poly(A)+ ribonucleoprotein complexes that induce a PKR-mediated deficient cell stress response
Goggin K, Beaudoin S, Grenier C, Brown AA, Roucou X
Biochim Biophys Acta. 1783(3):479-491
PMID: 18023289
Aggregation of cellular prion protein is initiated by proximity-induced dimerization
Goggin K, Bissonnette C, Grenier C, Volkov L, Roucou X
J Neurochem. 2007 Aug;102(4):1195-205
PMID: 17663754
The prion protein does not hesitate to produce aggregates
Goggin K, Roucou X
Med Sci (Paris). 22(12):1013-1014. French. No abstract available
PMID: 17156713
Prion protein prevents Bax-mediated cell death in the absence of other Bcl-2 family members in Saccharomyces cerevisiae
Bounhar Y, Mann KK, Roucou X, LeBlanc AC
FEMS Yeast Res. 2006 Dec;6(8):1204-1212
PMID: 17156017
Molecular morphology and toxicity of cytoplasmic prion protein aggregates in neuronal and non-neuronal cells
Grenier C, Bissonnette C, Volkov L, Roucou X
J Neurochem. 97(5):1456-1466
PMID: 16696854
Cellular prion protein inhibits proapoptotic Bax conformational change in human neurons and in breast carcinoma MCF-7 cells
Roucou X, Giannopoulos PN, Zhang Y, Jodoin J, Goodyer CG, LeBlanc A
Cell Death Differ. 12(7):783-795
PMID: 15846375
Cellular prion protein neuroprotective function: implications in prion diseases
Roucou X, LeBlanc AC
J Mol Med (Berl).83(1):3-11. Review
PMID: 15645198
Neuroprotective functions of prion protein
Roucou X, Gains M, LeBlanc AC
J Neurosci Res. 75(2):153-161. Review
PMID: 14705136
Cytosolic prion protein is not toxic and protects against Bax-mediated cell death in human primary neurons
Roucou X, Guo Q, Zhang Y, Goodyer CG, LeBlanc AC
J Biol Chem. 278(42):40877-40881
PMID: 12917444
p75 neurotrophin receptor protects primary cultures of human neurons against extracellular amyloid beta peptide cytotoxicity
Zhang Y, Hong Y, Bounhar Y, Blacker M, Roucou X, Tounekti O, Vereker E, Bowers WJ, Federoff HJ, Goodyer CG, LeBlanc A
J Neurosci. 23(19):7385-7394.
PMID: 12917374
Toxicity and protection in prions
LeBlanc AC, Roucou X
Science. 301(5630):168-169
PMID: 12855791
The molecular neighborhood of subunit 8 of yeast mitochondrial F1F0-ATP synthase probed by cysteine scanning mutagenesis and chemical modification
Stephens AN, Khan MA, Roucou X, Nagley P, Devenish RJ
J Biol Chem. 278(20):17867-17875.
PMID: 12626501
On the release of cytochrome c from mitochondria during cell death signaling
Lim ML, Lum MG, Hansen TM, Roucou X, Nagley P
J Biomed Sci. 9(6 Pt 1):488-506. Review
PMID: 12372987
Bax oligomerization in mitochondrial membranes requires tBid (caspase-8-cleaved Bid) and a mitochondrial protein
Roucou X, Montessuit S, Antonsson B, Martinou JC
Biochem J. 368(Pt 3):915-921
PMID: 12193163
Bid induces cytochrome c-impermeable Bax channels in liposomes
Roucou X, Rostovtseva T, Montessuit S, Martinou JC, Antonsson B
Biochem J. 363(Pt 3):547-552
PMID: 11964155
Involvement of mitochondria in apoptosis
Roucou X, Antonsson B, Martinou JC
Cardiol Clin. 19(1):45-55. Review
PMID: 11787813
Conformational change of Bax: a question of life or death
Roucou X, Martinou JC
Cell Death Differ. 8(9):875-877
PMID: 11526442
Expression of protein tyrosine phosphatase-like molecule ICA512/IA-2 induces growth arrest in yeast cells and transfected mammalian cell lines
Papakonstantinou T, Myers MA, Jois J, Roucou X, Prescott M, Rowley MJ, Mackay IR
J Autoimmun. 17(1):51-61.
PMID: 11488637
Modulation at a distance of proton conductance through the Saccharomyces cerevisiae mitochondrial F1F0-ATP synthase by variants of the oligomycin sensitivity-conferring protein containing substitutions near the C-terminus
Boyle GM, Roucou X, Nagley P, Devenish RJ, Prescott M
J Bioenerg Biomembr. 32(6):595-607
PMID: 15254373
Topology and proximity relationships of yeast mitochondrial ATP synthase subunit 8 determined by unique introduced cysteine residues
Stephens AN, Roucou X, Artika IM, Devenish RJ, Nagley P
Eur J Biochem. 267(21):6443-6451
PMID: 11029588
Insights into ATP synthase assembly and function through the molecular genetic manipulation of subunits of the yeast mitochondrial enzyme complex
Devenish RJ, Prescott M, Roucou X, Nagley P
Biochim Biophys Acta. 1458(2-3):428-442. Review
PMID: 10838056
A cytochrome c-GFP fusion is not released from mitochondria into the cytoplasm upon expression of Bax in yeast cells
Roucou X, Prescott M, Devenish RJ, Nagley P
FEBS Lett. 471(2-3):235-239
PMID: 10767430
Identification of subunit g of yeast mitochondrial F1F0-ATP synthase, a protein required for maximal activity of cytochrome c oxidase
Boyle GM, Roucou X, Nagley P, Devenish RJ, Prescott M
Eur J Biochem. 262(2):315-32
PMID: 10336613
Bioenergetic and structural consequences of allotopic expression of subunit 8 of yeast mitochondrial ATP synthase. The hydrophobic character of residues 23 and 24 is essential for maximal activity and structural stability of the enzyme complex
Roucou X, Artika IM, Devenish RJ, Nagley P
Eur J Biochem. 261(2):444-4512
PMID: 10215855
Characterization of the yeast mitochondria unselective channel: a counterpart to the mammalian permeability transition pore?
Manon S, Roucou X, Guérin M, Rigoulet M, Guérin B
J Bioenerg Biomembr. 30(5):419-429. Review
PMID: 9932645
Modulation of the electrophoretic ATP-induced K(+)-transport in yeast mitochondria by delta pH
Roucou X, Manon S, Guérin M
Biochem Mol Biol Int. 43(1):53-61
PMID: 9315282
Conditions allowing different states of ATP- and GDP-induced permeability in mitochondria from different strains of Saccharomyces cerevisiae
Roucou X, Manon S, Guérin M
Biochim Biophys Acta. 1324(1):120-132
PMID: 9059505
Stimulation of oxidative phosphorylation by electrophoretic K+ entry associated to electroneutral K+/H+ exchange in yeast mitochondria
Manon S, Roucou X, Rigoulet M, Guérin M
Biochim Biophys Acta. 1231(3):282-288
PMID: 7578216
Investigations of the inhibitory effect of propranolol, chlorpromazine, quinine, and dicyclohexylcarbodiimide on the swelling of yeast mitochondria in potassium acetate. Evidences for indirect effects mediated by the lipid phase
Roucou X, Manon S, Guérin M
J Bioenerg Biomembr. 27(3):353-362
PMID: 8847348
ATP opens an electrophoretic potassium transport pathway in respiring yeast mitochondria
Roucou X, Manon S, Guerin M
FEBS Lett. 364(2):161-164
PMID: 7750562